Radical S-Adenosyl Methionine Domain Containing 1 (RSAD1) (C-Term) antibody

Antigen: Radical S-Adenosyl Methionine Domain Containing 1 (RSAD1)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651455

Additional Information

Alternative name: RSAD1

Gene ID: 55316

UniProt: Q9HA92

Immunogen: This RSAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of human RSAD1.

Isotype: Ig

Description: Other names: Oxygen-independent coproporphyrinogen-III oxidase-like protein RSAD1, Radical S-adenosyl methionine domain-containing protein 1, mitochondrial,RSAD1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RSAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of human RSAD1.

Molecular Weight: 48714 DA

Comments:

Background: RSAD1 may be involved in porphyrin cofactor biosynthesis.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Metabolism

Restrictions: For Research Use only

Images

Western blot analysis of RSAD1 Antibody (C-term) (ABIN651455) in HepG2,NCI-H460 cell line lysates (35ug/lane).RSAD1 (arrow) was detected using the purified Pab.

RSAD1 Antibody (C-term) (ABIN651455) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RSAD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

RSAD1 Antibody (C-term) (ABIN651455) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Suzuki, Yoshitomo-Nakagawa, Maruyama et al.: "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library." in: Gene, Vol. 200, Issue 1-2, pp. 149-56, 1997 (PubMed).