Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1) (C-Term) antibody

Antigen: Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1)

Synonyms: PACAP, MGC126852, Pacap, adcyap1, GRF-PACAP, zgc:103760, xx:af217251, grf, ADCYAP1, MGC140674, pac1, pacapr, pacapri, MGC145986, pacap, Drm-amn, EP(X)1442, PACAP-38, PACAP-like, PACAP38, PACAP38-like, chpd, DmelCG11937, CG11937, pERp1, FLJ32987, AV064572, MGC73549, MGC112758, MGC159584

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN651855

Additional Information

Alternative name: ADCYAP1

Gene ID: 116

UniProt: P18509, NP_001093203.1

Immunogen: This ADCYAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-184 amino acids from the C-terminal region of human ADCYAP1.

Isotype: Ig

Description: Other names: Pituitary adenylate cyclase-activating polypeptide,ADCYAP1,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ADCYAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-176 amino acids from the C-terminal region of human ADCYAP1.

Molecular Weight: 18835 DA

Comments:

Background: ADCYAP1 is adenylate cyclase activating polypeptide 1. Mediated by adenylate cyclase activating polypeptide 1 receptors, this polypeptide stimulates adenylate cyclase and subsequently increases the cAMP level in target cells. Adenylate cyclase activating polypeptide 1 is not only a hypophysiotropic hormone, but also functions as a neurotransmitter and neuromodulator. In addition, it plays a role in paracrine and autocrine regulation of certain types of cells. This gene encodes three different mature peptides, including two isotypes, a shorter form and a longer form.

Synonyms: PACAP, MGC126852, Pacap, adcyap1, GRF-PACAP, zgc:103760, xx:af217251, grf, ADCYAP1, MGC140674, pac1, pacapr, pacapri, MGC145986, pacap, Drm-amn, EP(X)1442, PACAP-38, PACAP-like, PACAP38, PACAP38-like, chpd, DmelCG11937, CG11937, pERp1, FLJ32987, AV064572, MGC73549, MGC112758, MGC159584

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

ADCYAP1 Antibody (C-term) (ABIN651855) western blot analysis in 293 cell line lysates (35ug/lane). This demonstrates the ADCYAP1 antibody detected the ADCYAP1 protein (arrow).

ADCYAP1 Antibody (C-term) (ABIN651855) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADCYAP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ADCYAP1 Antibody (C-term) (ABIN651855) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Hosoya, Kimura, Ogi et al.: "Structure of the human pituitary adenylate cyclase activating polypeptide (PACAP) gene." in: Biochimica et biophysica acta, Vol. 1129, Issue 2, pp. 199-206, 1992 (PubMed).

Ohkubo, Kimura, Ogi et al.: "Primary structure and characterization of the precursor to human pituitary adenylate cyclase activating polypeptide." in: DNA and cell biology, Vol. 11, Issue 1, pp. 21-30, 1992 (PubMed).

Kimura, Ohkubo, Ogi et al.: "A novel peptide which stimulates adenylate cyclase: molecular cloning and characterization of the ovine and human cDNAs." in: Biochemical and biophysical research communications, Vol. 166, Issue 1, pp. 81-9, 1990 (PubMed).