Glutamate Receptor, Metabotropic 3 (GRM3) (C-Term) antibody

Antigen: Glutamate Receptor, Metabotropic 3 (GRM3)

Synonyms: GLUR3, mGlu3, GPRC1C, MGLUR3, mGluR3, Gprc1c, 0710001G23Rik, GRM3, DKFZp459N088

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652205

Additional Information

Alternative name: Metabotropic Glutamate Receptor 3 (GPRC1C)

Gene ID: 2913

UniProt: Q14832

Immunogen: This GPRC1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 833~863 amino acids from the C-terminal region of human GPRC1C.

Isotype: Ig

Description: Other names: Metabotropic glutamate receptor 3 [Precursor], mGluR3, GRM3, GPRC1C, MGLUR3,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This Metabotropic Glutamate Receptor 3 Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 833~863 amino acids from the C-terminal region of human GPRC1C.

Molecular Weight: 98879 DA

Comments:

Background: L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 (also known as GPRC1C) while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. The activity of GRM3 is mediated by a G-protein that inhibits adenylate cyclase activity.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Cell Signaling, Neurology, Cell Structure

Restrictions: For Research Use only

Images

GPRC1C Antibody (R841) (ABIN652205) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the GPRC1C antibody detected the GPRC1C protein (arrow).

Publications

Product: Scherer, Cheung, MacDonald et al.: "Human chromosome 7: DNA sequence and biology." in: Science (New York, N.Y.), Vol. 300, Issue 5620, pp. 767-72, 2003 (PubMed).

Aronica, Gorter, Ijlst-Keizers et al.: "Expression and functional role of mGluR3 and mGluR5 in human astrocytes and glioma cells: opposite regulation of glutamate transporter proteins." in: The European journal of neuroscience, Vol. 17, Issue 10, pp. 2106-18, 2003 (PubMed).

Yao, Koo, Wells et al.: "Expression of a truncated secreted form of the mGluR3 subtype of metabotropic glutamate receptor." in: Biochemical and biophysical research communications, Vol. 319, Issue 2, pp. 622-8, 2004 (PubMed).

Egan, Straub, Goldberg et al.: "Variation in GRM3 affects cognition, prefrontal glutamate, and risk for schizophrenia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 34, pp. 12604-9, 2004 (PubMed).

Aronica, Gorter, Rozemuller et al.: "Activation of metabotropic glutamate receptor 3 enhances interleukin (IL)-1beta-stimulated release of IL-6 in cultured human astrocytes." in: Neuroscience, Vol. 130, Issue 4, pp. 927-33, 2005 (PubMed).