Carbonic Anhydrase VB, Mitochondrial (CA5B) (C-Term) antibody

Antigen: Carbonic Anhydrase VB, Mitochondrial (CA5B)

Synonyms: CA-VB, MGC39962, CAV, Car5, CAVB, CarVb, 7330410H16Rik, D730005F19Rik, Ca5b, MGC93720, CA5B, zgc:171653

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN652315

Additional Information

Alternative name: CA5B

Gene ID: 11238

UniProt: Q9Y2D0

Immunogen: This CA5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261~290 amino acids from the C-terminal region of human CA5B.

Isotype: Ig

Description: Other names: Carbonic anhydrase 5B, mitochondrial, Carbonic anhydrase VB, CA-VB, Carbonate dehydratase VB, CA5B

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CA5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261~290 amino acids from the C-terminal region of human CA5B.

Molecular Weight: 36434 DA

Comments:

Background: CA5B belongs a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. This protein is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. The protein has a wider tissue distribution than CA VA, which is restricted to the liver.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CA5B Antibody (C-term) (ABIN652315) in mouse liver tissue lysates (35ug/lane). CA5B (arrow) was detected using the purified Pab.

CA5B Antibody (C-term) (ABIN652315) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CA5B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

CA5B Antibody (C-term) (ABIN652315) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Fujikawa-Adachi, Nishimori, Taguchi et al.: "Human mitochondrial carbonic anhydrase VB. cDNA cloning, mRNA expression, subcellular localization, and mapping to chromosome x." in: The Journal of biological chemistry, Vol. 274, Issue 30, pp. 21228-33, 1999 (PubMed).