ADAM Metallopeptidase Domain 9 (ADAM9) (C-Term) antibody

Antigen: ADAM Metallopeptidase Domain 9 (ADAM9)

Synonyms: adam9-A, MDC9, ADAM-9, Adam9, zgc:101824, ADAM9, LOC100101585, MCMP, Mltng, KIAA0021, AU020942, mKIAA0021

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652337

Additional Information

Alternative name: ADAM9

Gene ID: 8754

UniProt: Q13443

Immunogen: This ADAM9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 711~741 amino acids from the C-terminal region of human ADAM9.

Isotype: Ig

Description: Other names: Cellular disintegrin-related protein, Meltrin-gamma, Myeloma cell metalloproteinase, Metalloprotease/disintegrin/cysteine-rich protein 9, A disintegrin and metalloproteinase domain 9, ADAM 9, ADAM9, KIAA0021, MCMP, MDC9, MLTNG

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ADAM9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 711~741 amino acids from the C-terminal region of human ADAM9.

Molecular Weight: 90556 DA

Comments:

Background: ADAM9 is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of ADAM9 Antibody (C-term) (ABIN652337) in MDA-MB231 cell line lysates (35ug/lane). ADAM9 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ADAM9 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

ADAM9 Antibody (C-term) (ABIN652337) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Weskamp, Krätzschmar, Reid et al.: "MDC9, a widely expressed cellular disintegrin containing cytoplasmic SH3 ligand domains." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 717-26, 1996 (PubMed).

McKie, Edwards, Dallas et al.: "Expression of members of a novel membrane linked metalloproteinase family (ADAM) in human articular chondrocytes." in: Biochemical and biophysical research communications, Vol. 230, Issue 2, pp. 335-9, 1997 (PubMed).

Hotoda, Koike, Sasagawa et al.: "A secreted form of human ADAM9 has an alpha-secretase activity for APP." in: Biochemical and biophysical research communications, Vol. 293, Issue 2, pp. 800-5, 2002 (PubMed).