|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Hemopoietic Cell Kinase (HCK) (N-Term) antibody
|Synonyms||JTK9, Bmk, AI849071, MGC18625, Hctk, HCK, laloo, MGC107870|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml) (Variants)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This HCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the N-terminal region of human HCK.|
|Description||Other names: Tyrosine-protein kinase HCK,HCK,|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This HCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the N-terminal region of human HCK.|
|Molecular Weight||59600 DA|
Background: HCK is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq].
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Restrictions||For Research Use only|
Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).
Kennah, Ringrose, Zhou et al.: "Identification of tyrosine kinase, HCK, and tumor suppressor, BIN1, as potential mediators of AHI-1 oncogene in primary and transformed CTCL cells." in: Blood, Vol. 113, Issue 19, pp. 4646-55, 2009 (PubMed).
Hassan, Suzu, Hiyoshi et al.: "Dys-regulated activation of a Src tyroine kinase Hck at the Golgi disturbs N-glycosylation of a cytokine receptor Fms." in: Journal of cellular physiology, Vol. 221, Issue 2, pp. 458-68, 2009 (PubMed).
|Hosts||Rabbit (33), Mouse (7), Rat (1)|
|Reactivities||Human (40), Mouse (Murine) (25), Rat (Rattus) (24), Chicken (19), Cow (Bovine) (19), Horse (Equine) (18), Sheep (Ovine) (18), Cat (Feline) (1), Dog (Canine) (1)|
|Applications||Western Blotting (WB) (23), ELISA (21), Immunofluorescence (IF) (19), Immunohistochemistry (IHC) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (3), N-Term (2), AA 1-80 (1), AA 22-80 (1), AA 29-43 (1), pTyr209, pSer211 (1), pTyr410 (1)|