Hemopoietic Cell Kinase (HCK) (N-Term) antibody

Antigen: Hemopoietic Cell Kinase (HCK)

Synonyms: JTK9, Bmk, AI849071, MGC18625, Hctk, HCK, laloo, MGC107870

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652384

Additional Information

Alternative name: HCK

Gene ID: 3055

UniProt: P08631, NP_002101

Immunogen: This HCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the N-terminal region of human HCK.

Isotype: Ig

Description: Other names: Tyrosine-protein kinase HCK,HCK,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the N-terminal region of human HCK.

Molecular Weight: 59600 DA

Comments:

Background: HCK is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

HCK Antibody (N-term) (ABIN652384) western blot analysis in mouse lung tissue lysates (15ug/lane).This demonstrates the HCK antibody detected HCK protein (arrow).

HCK Antibody (N-term) (ABIN652384) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HCK Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

HCK Antibody (N-term) (ABIN652384) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).

Kennah, Ringrose, Zhou et al.: "Identification of tyrosine kinase, HCK, and tumor suppressor, BIN1, as potential mediators of AHI-1 oncogene in primary and transformed CTCL cells." in: Blood, Vol. 113, Issue 19, pp. 4646-55, 2009 (PubMed).

Hassan, Suzu, Hiyoshi et al.: "Dys-regulated activation of a Src tyroine kinase Hck at the Golgi disturbs N-glycosylation of a cytokine receptor Fms." in: Journal of cellular physiology, Vol. 221, Issue 2, pp. 458-68, 2009 (PubMed).