Actinin, alpha 1 (ACTN1) (N-Term) antibody

Antigen: Actinin, alpha 1 (ACTN1)

Synonyms: FLJ40884, FLJ54432, Actn1a, 3110023F10Rik, ACTN1, MGC128689, MGC147142

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652451

Additional Information

Alternative name: ACTN1

Gene ID: 87

UniProt: P12814

Immunogen: This ACTN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human ACTN1.

Isotype: Ig

Description: Other names: Alpha-actinin-1, Alpha-actinin cytoskeletal isoform, Non-muscle alpha-actinin-1, F-actin cross-linking protein, ACTN1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ACTN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human ACTN1.

Molecular Weight: 103058 DA

Comments:

Background: ACTN1 is a F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

Western blot analysis of ACTN1 Antibody (N-term) (ABIN652451) in MDA-MB231 cell line lysates (35ug/lane). ACTN1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human breast carcinoma with ACTN1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of MDA-231 cells using ACTN1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Zhang, Lin, Neel et al.: "Phosphorylated alpha-actinin and protein-tyrosine phosphatase 1B coregulate the disassembly of the focal adhesion kinase x Src complex and promote cell migration." in: The Journal of biological chemistry, Vol. 281, Issue 3, pp. 1746-54, 2006 (PubMed).