ADH5 (Center) antibody

Antigen: ADH5 (Center)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652502

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Gene ID: 128

Swiss-Prot: P11766

Immunogen: This ADH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219~248 amino acids from the Center region of human ADH5.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Alcohol dehydrogenase class-3, Alcohol dehydrogenase class-III, Alcohol dehydrogenase 5, Alcohol dehydrogenase class chi chain, S-(hydroxymethyl)glutathione dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase, GSH-FDH, FALDH, FDH, FDH, ADHX, ADH5

Molecular Weight: 39724 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ADH5 Antibody (Center) (ABIN652502) in K562 cell line lysates (35ug/lane). ADH5 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADH5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Flow cytometric analysis of K562 cells using ADH5 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.