Interleukin 8 (IL8) (C-Term) antibody

Antigen: Interleukin 8 (IL8)

Synonyms: NAF, GCP1, LECT, LUCT, NAP1, CXCL8, GCP-1, LYNAP, MDNCF, MONAP, NAP-1, IL-8, IL8, K60, EMF-1, AMCF-I, il8, si:dkey-151b16.2, cxcli2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652552

Additional Information

Alternative name: IL8

Gene ID: 3576

UniProt: P10145

Immunogen: This IL8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70~99 amino acids from the C-terminal region of human IL8.

Isotype: Ig

Description: Other names: Interleukin-8, IL-8, C-X-C motif chemokine 8, Monocyte-derived neutrophil chemotactic factor, MDNCF, T-cell chemotactic factor, Neutrophil-activating protein 1, NAP-1, Protein 3-10C, Granulocyte chemotactic protein 1, GCP-1, Monocyte-derived neutrophil-activating peptide, MONAP, Emoctakin, MDNCF-a, IL8/NAP1 form I, GCP/IL-8 protein IV, Interleukin-8, IL-8(1-77), MDNCF-b, IL8/NAP1 form II, GCP/IL-8 protein II, (Ala-IL-8)77, IL-8(5-77), IL-8(6-77), Lymphocyte-derived neutrophil-activating factor, LYNAP, Neutrophil-activating factor, NAF, MDNCF-c, IL8/NAP1 form III, GCP/IL-8 protein I, (Ser-IL-8)72, IL-8(7-77), IL8/NAP1 form IV, GCP/IL-8 protein V, IL-8(8-77), IL8/NAP1 form V, GCP/IL-8 protein VI, IL-8(9-77), IL8/NAP1 form VI, GCP/IL-8 protein III , CXCL8, IL8

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This IL8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70~99 amino acids from the C-terminal region of human IL8.

Molecular Weight: 11098 DA

Comments:

Background: IL8 is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Restrictions: For Research Use only

Images

Western blot analysis of IL8 Antibody (C-term) (ABIN652552) in MDA-MB468 cell line lysates (35ug/lane). IL8 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with IL8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

IL8 Antibody (C-term) (ABIN652552) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Baggiolini, Clark-Lewis: "Interleukin-8, a chemotactic and inflammatory cytokine." in: FEBS letters, Vol. 307, Issue 1, pp. 97-101, 1992 (PubMed).

Holmes, Lee, Kuang et al.: "Structure and functional expression of a human interleukin-8 receptor." in: Science (New York, N.Y.), Vol. 253, Issue 5025, pp. 1278-80, 1991 (PubMed).