Galactose 3-O-Sulfotransferase 1 (GAL3ST1) (Center) antibody

Antigen: Galactose 3-O-Sulfotransferase 1 (GAL3ST1)

Synonyms: CST, Cst, Gcst, MGC140153

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652831

Additional Information

Alternative name: GAL3ST1

Gene ID: 9514

UniProt: Q99999

Immunogen: This GAL3ST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95~124 amino acids from the Center region of human GAL3ST1.

Isotype: Ig

Description: Other names: 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase, 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase, Cerebroside sulfotransferase, Galactosylceramide sulfotransferase, GalCer sulfotransferase, GAL3ST1, CST

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GAL3ST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95~124 amino acids from the Center region of human GAL3ST1.

Molecular Weight: 48764 DA

Comments:

Background: Sulfonation, an important step in the metabolism of many drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed by sulfotransferases. GAL3ST1 is galactosylceramide sulfotransferase which catalyzes the conversion between 3'-phosphoadenylylsulfate + a galactosylceramide to adenosine 3',5'-bisphosphate + galactosylceramide sulfate. Activity of this sulfotransferase is enhanced in renal cell carcinoma.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology

Restrictions: For Research Use only

Images

Western blot analysis of GAL3ST1 Antibody (Center) (ABIN652831) in mouse cerebellum tissue lysates (35ug/lane). GAL3ST1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with GAL3ST1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

GAL3ST1 Antibody (Center) (ABIN652831) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Siegrist, Jutzi, Steck et al.: "Age-dependent modulation of 3'-phosphoadenosine-5'-phosphosulfate-galactosylceramide sulfotransferase by lipids extracted from the microsomal membranes and artificial lipid mixtures." in: Biochimica et biophysica acta, Vol. 489, Issue 1, pp. 58-63, 1977 (PubMed).

Stein, Gieselmann, Kreysing et al.: "Cloning and expression of human arylsulfatase A." in: The Journal of biological chemistry, Vol. 264, Issue 2, pp. 1252-9, 1989 (PubMed).