Abhydrolase Domain Containing 12 (ABHD12) (N-Term) antibody

Antigen: Abhydrolase Domain Containing 12 (ABHD12)

Synonyms: ABHD12A, BEM46L2, C20orf22, dJ965G21.2, DKFZp434P106, AI431047, AW547313, 1500011G07Rik, 6330583M11Rik, RP23-241M12.2, ABHD12

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652851

Additional Information

Alternative name: ABHD12

Gene ID: 26090

UniProt: Q8N2K0

Immunogen: This ABHD12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46~75 amino acids from the N-terminal region of human ABHD12.

Isotype: Ig

Description: Other names: Abhydrolase domain-containing protein 12, 2-arachidonoylglycerol hydrolase, Monoacylglycerol lipase ABHD12, ABHD12, C20orf22

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ABHD12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46~75 amino acids from the N-terminal region of human ABHD12.

Molecular Weight: 45097 DA

Comments:

Background: ABHD12 has 2-arachidonoylglycerol hydrolase activity (By similarity). It may be a regulator of endocannabinoid signaling pathways (By similarity).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ABHD12 Antibody (N-term) (ABIN652851) in mouse cerebellum tissue lysates (35ug/lane). ABHD12 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with ABHD12 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

ABHD12 Antibody (N-term) (ABIN652851) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Bechtel, Rosenfelder, Duda et al.: "The full-ORF clone resource of the German cDNA Consortium." in: BMC genomics, Vol. 8, pp. 399, 2008 (PubMed).