Chromobox Homolog 1 (CBX1) (C-Term) antibody

Antigen: Chromobox Homolog 1 (CBX1)

Synonyms: CBX, M31, MOD1, p25beta, HP1-BETA, HP1Hsbeta, HP1Hs-beta

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN652861

Additional Information

Alternative name: CBX1

Gene ID: 10951

UniProt: P83916

Immunogen: This CBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135~164 amino acids from the C-terminal region of human CBX1.

Isotype: Ig

Description: Other names: p25beta, HP1Hsbeta, Heterochromatin protein p25, M31, Modifier 1 protein, Heterochromatin protein 1 homolog beta, HP1 beta, Chromobox protein homolog 1, CBX1, CBX

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135~164 amino acids from the C-terminal region of human CBX1.

Molecular Weight: 21418 DA

Comments:

Background: CBX1 is a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology

Restrictions: For Research Use only

Images

Western blot analysis of CBX1 Antibody (C-term) (ABIN652861) in MCF-7, A2058, Y79 cell line lysates (35ug/lane). CBX1 (arrow) was detected using the purified Pab.

CBX1 Antibody (C-term) (ABIN652861) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CBX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

CBX1 Antibody (C-term) (ABIN652861) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Aagaard, Laible, Selenko et al.: "Functional mammalian homologues of the Drosophila PEV-modifier Su(var)3-9 encode centromere-associated proteins which complex with the heterochromatin component M31." in: The EMBO journal, Vol. 18, Issue 7, pp. 1923-38, 1999 (PubMed).