Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (Center) antibody

Antigen: Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1)

Synonyms: DOD, UBL5, D9Bwg1161e, 0610025L01Rik, dod, ubl5, pin1b, PIN1, MGC89352, pin1a, MGC132291, MGC137201

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN652906

Additional Information

Alternative name: PIN1

Gene ID: 5300

UniProt: Q13526

Immunogen: This PIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36~66 amino acids from the Center region of human PIN1.

Isotype: Ig

Description: Other names: Rotamase Pin1, Peptidyl-prolyl cis-trans isomerase Pin1, PPIase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, PIN1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36~66 amino acids from the Center region of human PIN1.

Molecular Weight: 18243 DA

Comments:

Background: PIN1 is an essential nuclear peptidylprolyl cis-trans isomerase (PPIase, EC 5.2.1.8) involved in regulation of mitosis.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Cycle, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of PIN1 Antibody (Center) (ABIN652906) in HL-60 cell line lysates (35ug/lane). PIN1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PIN1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

PIN1 Antibody (Center) (ABIN652906) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Lu, Hanes, Hunter: "A human peptidyl-prolyl isomerase essential for regulation of mitosis." in: Nature, Vol. 380, Issue 6574, pp. 544-7, 1996 (PubMed).