Spindle and Kinetochore Associated Complex Subunit 3 (SKA3) (Center) antibody

Antigen: Spindle and Kinetochore Associated Complex Subunit 3 (SKA3)

Synonyms: RAMA1, C13orf3, MGC4832, RGD1307201, MGC137359, C12H13ORF3, fc23d11, wu:fc23d11, si:rp71-68n21.13, rama1

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN652928

Additional Information

Alternative name: C13orf3

Gene ID: 221150

UniProt: Q8IX90

Immunogen: This C13orf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232~262 amino acids from the Center region of human C13orf3.

Isotype: Ig

Description: Other names: Spindle and kinetochore-associated protein 3, SKA3, C13orf3, RAMA1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This C13orf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232~262 amino acids from the Center region of human C13orf3.

Molecular Weight: 46359 DA

Comments:

Background: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of C13orf3 Antibody (Center) (ABIN652928) in Hela, 293 cell line lysates (35ug/lane). C13orf3 (arrow) was detected using the purified Pab.

C13orf3 Antibody (Center) (ABIN652928) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C13orf3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

C13orf3 Antibody (Center) (ABIN652928) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).