CDC14 Cell Division Cycle 14 Homolog B (S. Cerevisiae) (CDC14B) (Center) antibody

Antigen: CDC14 Cell Division Cycle 14 Homolog B (S. Cerevisiae) (CDC14B)

Synonyms: CDC14B3, Cdc14B1, Cdc14B2, hCDC14B, AA472821, 2810432N10Rik, A530086E13Rik, CDC14B

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653024

Additional Information

Alternative name: CDC14B

Gene ID: 8555

UniProt: O60729

Immunogen: This CDC14B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271~301 amino acids from the Center region of human CDC14B.

Isotype: Ig

Description: Other names: CDC14 cell division cycle 14 homolog B, Dual specificity protein phosphatase CDC14B, CDC14B

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDC14B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271~301 amino acids from the Center region of human CDC14B.

Molecular Weight: 56802 DA

Comments:

Background: CDC14B is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Neurology

Restrictions: For Research Use only

Images

Western blot analysis of CDC14B Antibody (Center) (ABIN653024) in 293 cell line lysates (35ug/lane). CDC14B (arrow) was detected using the purified Pab.

CDC14B Antibody (Center) (ABIN653024) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Bassermann, Frescas, Guardavaccaro et al.: "The Cdc14B-Cdh1-Plk1 axis controls the G2 DNA-damage-response checkpoint." in: Cell, Vol. 134, Issue 2, pp. 256-67, 2008 (PubMed).