Acyl-CoA Thioesterase 8 (ACOT8) (C-Term) antibody

Antigen: Acyl-CoA Thioesterase 8 (ACOT8)

Synonyms: hTE, PTE1, PTE2, PTE-2, HNAACTE, hACTE-III, Pte1, zgc:101691, MGC140184

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653089

Additional Information

Alternative name: ACOT8

Gene ID: 10005

UniProt: O14734

Immunogen: This ACOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290~319 amino acids from the C-terminal region of human ACOT8.

Isotype: Ig

Description: Other names: PTE-2, Thioesterase II, hTE, hACTEIII, hACTE-III, HIV-Nef-associated acyl-CoA thioesterase, Peroxisomal long-chain acyl-CoA thioesterase 1, Peroxisomal acyl-coenzyme A thioester hydrolase 1, PTE-1, Acyl-CoA thioesterase 8, Choloyl-coenzyme A thioesterase, Acyl-coenzyme A thioesterase 8, PTE2, PTE1, ACTEIII, ACOT8

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ACOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290~319 amino acids from the C-terminal region of human ACOT8.

Molecular Weight: 35914 DA

Comments:

Background: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. It may mediate Nef-induced down-regulation of CD4. It may be involved in the metabolic regulation of peroxisome proliferation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ACOT8 Antibody (C-term) (ABIN653089) in mouse liver tissue lysates (35ug/lane). ACOT8 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human Prostate carcinoma reacted with ACOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

ACOT8 Antibody (C-term) (ABIN653089) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Daub, Olsen, Bairlein et al.: "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." in: Molecular cell, Vol. 31, Issue 3, pp. 438-48, 2008 (PubMed).

Choudhary, Kumar, Gnad et al.: "Lysine acetylation targets protein complexes and co-regulates major cellular functions." in: Science (New York, N.Y.), Vol. 325, Issue 5942, pp. 834-40, 2009 (PubMed).