Minichromosome Maintenance Complex Component 2 (MCM2) (C-Term) antibody

Antigen: Minichromosome Maintenance Complex Component 2 (MCM2)

Synonyms: BM28, CCNL1, CDCL1, cdc19, D3S3194, MITOTIN, KIAA0030, MGC10606, cb737, chunp6905, mcm2, MGC89529

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653324

Additional Information

Alternative name: MCM2

Gene ID: 4171

UniProt: P49736

Immunogen: This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656~685 amino acids from the C-terminal region of human MCM2.

Isotype: Ig

Description: Other names: Nuclear protein BM28, Minichromosome maintenance protein 2 homolog, DNA replication licensing factor MCM2, KIAA0030, CDCL1, CCNL1, BM28, MCM2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656~685 amino acids from the C-terminal region of human MCM2.

Molecular Weight: 101896 DA

Comments:

Background: MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of MCM2 Antibody (C-term) (ABIN653324) in HL-60 cell line lysates (35ug/lane). MCM2 (arrow) was detected using the purified Pab.

MCM2 Antibody (C-term) (ABIN653324) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCM2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Sugiyama, Masuda, Shinoda et al.: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, 2007 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Liaw, Boyd: "MCM 2 Expression in Basal Cell Carcinomas and Trichoepitheliomas." in: Journal of cutaneous pathology, Vol. 36, Issue 10, pp. 1121-2, 2009 (PubMed).

Chuang, Teixeira, Wohlschlegel et al.: "Phosphorylation of Mcm2 by Cdc7 promotes pre-replication complex assembly during cell-cycle re-entry." in: Molecular cell, Vol. 35, Issue 2, pp. 206-16, 2009 (PubMed).

Im, Ki, Farina et al.: "Assembly of the Cdc45-Mcm2-7-GINS complex in human cells requires the Ctf4/And-1, RecQL4, and Mcm10 proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 37, pp. 15628-32, 2009 (PubMed).