Adrenergic, alpha-1B-, Receptor (ADRA1B) (Center) antibody

Antigen: Adrenergic, alpha-1B-, Receptor (ADRA1B)

Synonyms: ADRA1B, LOC100136712, ADRA1, ALPHA1BAR, RDC5

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653353

Additional Information

Alternative name: ADRA1B

Gene ID: 147

UniProt: P35368

Immunogen: This ADRA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276~306 amino acids from the Central region of human ADRA1B.

Isotype: Ig

Description: Other names: Alpha-1B adrenergic receptor, Alpha-1B adrenoreceptor, ADRA1B

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ADRA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276~306 amino acids from the Central region of human ADRA1B.

Molecular Weight: 56,836 DA

Comments:

Background: Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This protein encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This protein comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ADRA1B Antibody (Center) (ABIN653353) in NCI-H460 cell line lysates (35ug/lane). ADRA1B (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain with ADRA1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Publications

Product: Mathias, Grant, Rafaels et al.: "A genome-wide association study on African-ancestry populations for asthma." in: The Journal of allergy and clinical immunology, Vol. 125, Issue 2, pp. 336-346.e4, 2010 (PubMed).