F-Box and Leucine-Rich Repeat Protein 5 (FBXL5) (N-Term) antibody

Antigen: F-Box and Leucine-Rich Repeat Protein 5 (FBXL5)

Synonyms: FBL4, FBL5, FLR1, Fbl4, Fir4, wu:fb52d06, fbxl5, MGC53238, MGC81139, FBXL5, MGC148944, DKFZp459A167

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653358

Additional Information

Alternative name: FBXL5

Gene ID: 26234

UniProt: Q9UKA1

Immunogen: This FBXL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93~123 amino acids from the N-terminal region of human FBXL5.

Isotype: Ig

Description: Other names: p45SKP2-like protein, F-box protein FBL4/FBL5, F-box and leucine-rich repeat protein 5, F-box/LRR-repeat protein 5, FLR1, FBL5, FBL4, FBXL5

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FBXL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93~123 amino acids from the N-terminal region of human FBXL5.

Molecular Weight: 78,555 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of FBXL5 Antibody (N-term) (ABIN653358) in HepG2 cell line lysates (35ug/lane). FBXL5 (arrow) was detected using the purified Pab.

Western blot analysis of FBXL5 Antibody (N-term) (ABIN653358) in mouse heart tissue lysates (35ug/lane). FBXL5 (arrow) was detected using the purified Pab.

FBXL5 Antibody (N-term) (ABIN653358) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FBXL5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Ilyin, Rialland, Pigeon et al.: "cDNA cloning and expression analysis of new members of the mammalian F-box protein family." in: Genomics, Vol. 67, Issue 1, pp. 40-7, 2000 (PubMed).

Zhang, Liu, Ding et al.: "FBXL5 interacts with p150Glued and regulates its ubiquitination." in: Biochemical and biophysical research communications, Vol. 359, Issue 1, pp. 34-9, 2007 (PubMed).

Vashisht, Zumbrennen, Huang et al.: "Control of iron homeostasis by an iron-regulated ubiquitin ligase." in: Science (New York, N.Y.), Vol. 326, Issue 5953, pp. 718-21, 2009 (PubMed).

Salahudeen, Thompson, Ruiz et al.: "An E3 ligase possessing an iron-responsive hemerythrin domain is a regulator of iron homeostasis." in: Science (New York, N.Y.), Vol. 326, Issue 5953, pp. 722-6, 2009 (PubMed).