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CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA) (C-Term) antibody
Alternatives Western Blotting (WB), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This CEBPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310~339 amino acids from the C-terminal region of human CEBPA.|
|Description||Other names: CCAAT/enhancer-binding protein alpha, C/EBP alpha, CEBPA|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This CEBPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310~339 amino acids from the C-terminal region of human CEBPA.|
|Molecular Weight||37561 DA|
Background: CEBPA is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. This protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Signaling, Protein Modifications, Transcription Factors|
|Restrictions||For Research Use only|
|Western blot analysis of CEBPA Antibody (C-term) (ABIN653431) in mouse liver tissue lysates (35ug/lane). CEBPA (arrow) was detected using the purified Pab.|
Nanri, Uike, Kawakita et al.: "A family harboring a germ-line N-terminal C/EBPalpha mutation and development of acute myeloid leukemia with an additional somatic C-terminal C/EBPalpha mutation." in: Genes, chromosomes & cancer, Vol. 49, Issue 3, pp. 237-41, 2010 (PubMed).
Burnett, Hills, Green et al.: "The impact on outcome of the addition of all-trans retinoic acid to intensive chemotherapy in younger patients with nonacute promyelocytic acute myeloid leukemia: overall results and results in genotypic subgroups defined by mutations in NPM1, FLT3, and C" in: Blood, Vol. 115, Issue 5, pp. 948-56, 2010 (PubMed).
Dufour, Schneider, Metzeler et al.: "Acute myeloid leukemia with biallelic CEBPA gene mutations and normal karyotype represents a distinct genetic entity associated with a favorable clinical outcome." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 28, Issue 4, pp. 570-7, 2010 (PubMed).
|Hosts||Rabbit (56), Mouse (9)|
|Reactivities||Human (62), Rat (Rattus) (46), Mouse (Murine) (44), Cow (Bovine) (31), Pig (Porcine) (29), Dog (Canine) (25), Chicken (7), Cat (Feline) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (37), Immunofluorescence (IF) (28), ELISA (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Flow Cytometry (FACS) (7), Immunoprecipitation (IP) (7), Immunohistochemistry (IHC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Dot Blot (DB) (1), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)|
|Epitopes||pSer21 (7), N-Term (4), pThr226,pThr222 (4), C-Term (3), Internal Region (2), pSer69 (1), pThr226 (1)|