CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA) (C-Term) antibody

Antigen: CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653431

Additional Information

Alternative name: CEBPA

Gene ID: 1050

UniProt: P49715

Immunogen: This CEBPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310~339 amino acids from the C-terminal region of human CEBPA.

Isotype: Ig

Description: Other names: CCAAT/enhancer-binding protein alpha, C/EBP alpha, CEBPA

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CEBPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310~339 amino acids from the C-terminal region of human CEBPA.

Molecular Weight: 37561 DA

Comments:

Background: CEBPA is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. This protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of CEBPA Antibody (C-term) (ABIN653431) in mouse liver tissue lysates (35ug/lane). CEBPA (arrow) was detected using the purified Pab.

Publications

Product: Nanri, Uike, Kawakita et al.: "A family harboring a germ-line N-terminal C/EBPalpha mutation and development of acute myeloid leukemia with an additional somatic C-terminal C/EBPalpha mutation." in: Genes, chromosomes & cancer, Vol. 49, Issue 3, pp. 237-41, 2010 (PubMed).

Burnett, Hills, Green et al.: "The impact on outcome of the addition of all-trans retinoic acid to intensive chemotherapy in younger patients with nonacute promyelocytic acute myeloid leukemia: overall results and results in genotypic subgroups defined by mutations in NPM1, FLT3, and C" in: Blood, Vol. 115, Issue 5, pp. 948-56, 2010 (PubMed).

Dufour, Schneider, Metzeler et al.: "Acute myeloid leukemia with biallelic CEBPA gene mutations and normal karyotype represents a distinct genetic entity associated with a favorable clinical outcome." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 28, Issue 4, pp. 570-7, 2010 (PubMed).