CEBPA antibody (AA 288-337)

Catalog No. ABIN604772

The Rabbit Polyclonal anti-CEBPA antibody is suitable to detect CEBPA in samples from Human, Rat, Mouse, Cow, Guinea Pig, Pig, Chicken, Dog and Rabbit. It has been validated for WB, IHC and IHC (p).

Quick Overview for CEBPA antibody (AA 288-337) (ABIN604772)

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Reactivity: Human, Rat, Mouse, Cow, Guinea Pig, Pig, Chicken, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CEBPA Antibody

Binding Specificity: AA 288-337

Specificity: Human CEBPA / CEBP Alpha

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig, Guinea pig, Chicken (100%) Xenopus, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa288-337 of human CEBPA (P49715, NP_004355). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Pig, Guinea pig, Zebra finch, Chicken, Platypus (100%), Marmoset, Xenopus, Trout, Salmon, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CEBPA

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Alternative Name: C/EBP Alpha / CEBPA

Background: Name/Gene ID: CEBPA

Synonyms: CEBPA, C/EBP alpha, C/EBP-alpha, CEBP

Gene ID: 1050

NCBI Accession: NP_004355

UniProt: P49715

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation