AF4/FMR2 Family, Member 4 (AFF4) (N-Term) antibody

Antigen: AF4/FMR2 Family, Member 4 (AFF4)

Synonyms: MCEF, AF5Q31, MGC75036, Alf4, Laf4l, MGC107469, AFF4, RA_m002_jsmFBA6Br

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653641

Additional Information

Alternative name: AF5q31

Gene ID: 27125

UniProt: Q9UHB7

Immunogen: This AF5q31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human AF5q31.

Isotype: Ig

Description: Other names: Major CDK9 elongation factor-associated protein, ALL1-fused gene from chromosome 5q31 protein, Protein AF-5q31, AF4/FMR2 family member 4, HSPC092, MCEF, AF5Q31, AFF4

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This AF5q31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human AF5q31.

Molecular Weight: 127459 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of AF5q31 Antibody (N-term) (ABIN653641) in HL-60 cell line lysates (35ug/lane). AF5q31 (arrow) was detected using the purified Pab.

Publications

Product: Sugiyama, Masuda, Shinoda et al.: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, 2007 (PubMed).

Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Lin, Smith, Takahashi et al.: "AFF4, a component of the ELL/P-TEFb elongation complex and a shared subunit of MLL chimeras, can link transcription elongation to leukemia." in: Molecular cell, Vol. 37, Issue 3, pp. 429-37, 2010 (PubMed).