TM173 (C-Term) antibody

Antigen: TM173

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653703

Additional Information

Gene ID: 340061

UniProt: Q86WV6

Immunogen: This TM173 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318~348 amino acids from the C-terminal region of human TM173.

Isotype: Ig

Description: Other names: Mediator of IRF3 activation, hMITA, Stimulator of interferon genes protein, hSTING, Transmembrane protein 173, STING, MITA, TMEM173

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TM173 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318~348 amino acids from the C-terminal region of human TM173.

Molecular Weight: 42193 DA

Comments:

Background: Acts as a facilitator of innate immune signaling. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon (IFN-alpha and IFN-beta) and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of TM173 Antibody (C-term) (ABIN653703) in mouse spleen tissue lysates (35ug/lane). TM173 (arrow) was detected using the purified Pab.

TM173 Antibody (C-term) (ABIN653703) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Zhong, Zhang, Lei et al.: "The ubiquitin ligase RNF5 regulates antiviral responses by mediating degradation of the adaptor protein MITA." in: Immunity, Vol. 30, Issue 3, pp. 397-407, 2009 (PubMed).

Sun, Li, Chen et al.: "ERIS, an endoplasmic reticulum IFN stimulator, activates innate immune signaling through dimerization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 21, pp. 8653-8, 2009 (PubMed).