ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A (ATP6V1A) (Center) antibody

Antigen: ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A (ATP6V1A)

Synonyms: HO68, VA68, VPP2, Vma1, ATP6A1, ATP6V1A1, Atp6a1, Atp6a2, AI647066, Atp6v1a1, ATP6V1A, MGC126942, MGC63516, zgc:63516, DKFZp459K064

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653735

Additional Information

Alternative name: ATP6V1A

Gene ID: 523

UniProt: P38606

Immunogen: This ATP6V1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 447~477 amino acids from the Central region of human ATP6V1A.

Isotype: Ig

Description: Other names: Vacuolar ATPase isoform VA68, V-ATPase 69 kDa subunit, Vacuolar proton pump subunit alpha, V-type proton ATPase catalytic subunit A, V-ATPase subunit A,VPP2, ATP6V1A1, ATP6A1, ATP6V1A

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ATP6V1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 447~477 amino acids from the Central region of human ATP6V1A.

Molecular Weight: 68304 DA

Comments:

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cancer, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ATP6V1A Antibody (Center) (ABIN653735) in A2058 cell line lysates (35ug/lane). ATP6V1A (arrow) was detected using the purified Pab.

ATP6V1A Antibody (Center) (ABIN653735) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP6V1A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Morel: "Neurotransmitter release: the dark side of the vacuolar-H+ATPase." in: Biology of the cell / under the auspices of the European Cell Biology Organization, Vol. 95, Issue 7, pp. 453-7, 2003 (PubMed).

Ahmed, Forsberg, Bergsten: "Protein profiling of human pancreatic islets by two-dimensional gel electrophoresis and mass spectrometry." in: Journal of proteome research, Vol. 4, Issue 3, pp. 931-40, 2005 (PubMed).

Martins-de-Souza, Gattaz, Schmitt et al.: "Proteomic analysis of dorsolateral prefrontal cortex indicates the involvement of cytoskeleton, oligodendrocyte, energy metabolism and new potential markers in schizophrenia." in: Journal of psychiatric research, Vol. 43, Issue 11, pp. 978-86, 2009 (PubMed).