ATP6V1A antibody (AA 441-468)

Catalog No. ABIN653735

There is 1 publication for this product available. The Rabbit Polyclonal anti-ATP6V1A antibody is suitable to detect ATP6V1A in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for ATP6V1A antibody (AA 441-468) (ABIN653735)

Target: ATP6V1A (ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A (ATP6V1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB24578

Product Details anti-ATP6V1A Antibody

Binding Specificity: AA 441-468

Predicted Reactivity: B, M, Pig

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATP6V1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 441-468 amino acids from the Central region of human ATP6V1A.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ATP6V1A antibody (ABIN653735)

Nabatov, Hatzis, Rouschop, van Diest, Vooijs: "Hypoxia inducible NOD2 interacts with 3-O-sulfogalactoceramide and regulates vesicular homeostasis." in: Biochimica et biophysica acta, Vol. 1830, Issue 11, pp. 5277-86, (2013) (PubMed).

Target Details for ATP6V1A

Target: ATP6V1A (ATPase, H+ Transporting, Lysosomal 70kDa, V1 Subunit A (ATP6V1A))

Alternative Name: ATP6V1A

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues.

Molecular Weight: 68304

Gene ID: 523

NCBI Accession: NP_001681

UniProt: P38606

Pathways: Transition Metal Ion Homeostasis, Proton Transport, SARS-CoV-2 Protein Interactome