ADAM Metallopeptidase Domain 19 (Adam19) (Center) antibody

Antigen: ADAM Metallopeptidase Domain 19 (Adam19)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653772

Additional Information

Alternative name: ADAM19

Gene ID: 8728

UniProt: Q9H013

Immunogen: This ADAM19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215~245 amino acids from the Central region of human ADAM19.

Isotype: Ig

Description: Other names: Metalloprotease and disintegrin dendritic antigen marker, MADDAM, Meltrin-beta, Disintegrin and metalloproteinase domain-containing protein 19, ADAM 19, FKSG34, MLTNB, ADAM19

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ADAM19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215~245 amino acids from the Central region of human ADAM19.

Molecular Weight: 105039 DA

Comments:

Background: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has also been demonstrated to be an active metalloproteinase, which may be involved in normal physiological and pathological processes such as cells migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ADAM19 Antibody (Center) (ABIN653772) in WiDr cell line lysates (35ug/lane). ADAM19 (arrow) was detected using the purified Pab.

ADAM19 Antibody (Center) (ABIN653772) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADAM19 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

ADAM19 Antibody (Center) (ABIN653772) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Tanabe, Hotoda, Sasagawa et al.: "ADAM19 is tightly associated with constitutive Alzheimer's disease APP alpha-secretase in A172 cells." in: Biochemical and biophysical research communications, Vol. 352, Issue 1, pp. 111-7, 2006 (PubMed).

Hancock, Eijgelsheim, Wilk et al.: "Meta-analyses of genome-wide association studies identify multiple loci associated with pulmonary function." in: Nature genetics, Vol. 42, Issue 1, pp. 45-52, 2009 (PubMed).