Zinc Finger, RAN-Binding Domain Containing 1 (ZRANB1) (C-Term) antibody

Antigen: Zinc Finger, RAN-Binding Domain Containing 1 (ZRANB1)

Synonyms: TRABID, DKFZp762P2216, Gm1956, Trabid, D7Wsu87e, 9330160G10Rik, zranb1-a, zranb1, zgc:154177, trabid

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653787

Additional Information

Alternative name: ZRANB1

Gene ID: 54764

UniProt: Q9UGI0

Immunogen: This ZRANB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672~701 amino acids from the C-terminal region of human ZRANB1.

Isotype: Ig

Description: Other names: hTrabid, Zinc finger Ran-binding domain-containing protein 1, Ubiquitin thioesterase ZRANB1,TRABID, ZRANB1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ZRANB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 672~701 amino acids from the C-terminal region of human ZRANB1.

Molecular Weight: 80967 DA

Comments:

Background: ZRANB1 is a positive regulator of the Wnt signaling pathway that specifically cleaves 'Lys-63'-linked ubiquitin chains. It acts by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription and may also modulate TNF-alpha signaling.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Cell Signaling, Protein Modifications, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of ZRANB1 Antibody (C-term) (ABIN653787) in K562 cell line lysates (35ug/lane). ZRANB1 (arrow) was detected using the purified Pab.

Western blot analysis of ZRANB1 Antibody (C-term) (ABIN653787) in mouse cerebellum tissue lysates (35ug/lane). ZRANB1 (arrow) was detected using the purified Pab.

ZRANB1 Antibody (C-term) (ABIN653787) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZRANB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Tran, Hamada, Schwarz-Romond et al.: "Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains." in: Genes & development, Vol. 22, Issue 4, pp. 528-42, 2008 (PubMed).