CDC45 antibody (AA 236-263)

Catalog No. ABIN653837

The Rabbit Polyclonal anti-CDC45 antibody has been validated for WB, FACS and IHC (p). It is suitable to detect CDC45 in samples from Human.

Quick Overview for CDC45 antibody (AA 236-263) (ABIN653837)

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC45 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB23958

Product Details anti-CDC45 Antibody

Binding Specificity: AA 236-263

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CDC45L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-263 amino acids from the Central region of human CDC45L.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for CDC45

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Alternative Name: CDC45L

Background: The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.

Molecular Weight: 65569

Gene ID: 8318

NCBI Accession: NP_001171481, NP_001171482, NP_003495

UniProt: O75419

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA