|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (Center) antibody
|Synonyms||CDC45, CDC45L2, PORC-PI-1, cdc45l, cdc45l2, porc-pi-1, MGC84655|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This CDC45L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242~272 amino acids from the Central region of human CDC45L.|
|Description||Other names: PORC-PI-1, CDC45-related protein, Cdc45,UNQ374/PRO710, CDC45L2, CDC45L|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This CDC45L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242~272 amino acids from the Central region of human CDC45L.|
|Molecular Weight||65569 DA|
Background: The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Phospho-specific antibodies, Protein Modifications, Chromatin, Signaling, Neurology, Cell Structure|
|Restrictions||For Research Use only|
Hoskins, Marcuello, Altes et al.: "Irinotecan pharmacogenetics: influence of pharmacodynamic genes." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 6, pp. 1788-96, 2008 (PubMed).
Ballabeni, Zamponi, Caprara et al.: "Human CDT1 associates with CDC7 and recruits CDC45 to chromatin during S phase." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 3028-36, 2009 (PubMed).
Enjuanes, Benavente, Bosch et al.: "Genetic variants in apoptosis and immunoregulation-related genes are associated with risk of chronic lymphocytic leukemia." in: Cancer research, Vol. 68, Issue 24, pp. 10178-86, 2008 (PubMed).
|Hosts||Rabbit (28), Mouse (5)|
|Reactivities||Human (32), Mouse (Murine) (23), Rat (Rattus) (23), Horse (Equine) (17), Pig (Porcine) (17), Chicken (2), Cow (Bovine) (2), Dog (Canine) (2), Cat (Feline) (1), Xenopus laevis (1)|
|Applications||Western Blotting (WB) (16), Immunofluorescence (IF) (15), ELISA (8), Immunohistochemistry (IHC) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Flow Cytometry (FACS) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (2), Internal Region,AA 242-272 (1)|