Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45) (Center) antibody

Antigen: Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45)

Synonyms: CDC45, CDC45L2, PORC-PI-1, cdc45l, cdc45l2, porc-pi-1, MGC84655

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN653837

Additional Information

Alternative name: CDC45L

Gene ID: 8318

UniProt: O75419

Immunogen: This CDC45L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242~272 amino acids from the Central region of human CDC45L.

Isotype: Ig

Description: Other names: PORC-PI-1, CDC45-related protein, Cdc45,UNQ374/PRO710, CDC45L2, CDC45L

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This CDC45L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242~272 amino acids from the Central region of human CDC45L.

Molecular Weight: 65569 DA

Comments:

Background: The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple polyadenlyation sites of this gene are reported.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Protein Modifications, Chromatin, Signaling, Neurology, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of CDC45L Antibody (Center) (ABIN653837) in 293 cell line lysates (35ug/lane). CDC45L (arrow) was detected using the purified Pab.

CDC45L Antibody (Center) (ABIN653837) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CDC45L Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

CDC45L Antibody (Center) (ABIN653837) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Hoskins, Marcuello, Altes et al.: "Irinotecan pharmacogenetics: influence of pharmacodynamic genes." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 6, pp. 1788-96, 2008 (PubMed).

Ballabeni, Zamponi, Caprara et al.: "Human CDT1 associates with CDC7 and recruits CDC45 to chromatin during S phase." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 3028-36, 2009 (PubMed).

Enjuanes, Benavente, Bosch et al.: "Genetic variants in apoptosis and immunoregulation-related genes are associated with risk of chronic lymphocytic leukemia." in: Cancer research, Vol. 68, Issue 24, pp. 10178-86, 2008 (PubMed).