IMP3, U3 Small Nucleolar Ribonucleoprotein, Homolog (Yeast) (IMP3) (C-Term) antibody

Antigen: IMP3, U3 Small Nucleolar Ribonucleoprotein, Homolog (Yeast) (IMP3)

Synonyms: BRMS2, MRPS4, C15orf12, FLJ10968, DKFZp586L0118, AI256594, 1190002L16Rik, RGD1306825, MGC56526, zgc:56526

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653856

Additional Information

Alternative name: IMP3

Gene ID: 55272

UniProt: Q9NV31

Immunogen: This IMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155~184 amino acids from the C-terminal region of human IMP3.

Isotype: Ig

Description: Other names: BRMS2, U3 small nucleolar ribonucleoprotein protein IMP3, U3 snoRNP protein IMP3,MRPS4, C15orf12, IMP3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This IMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155~184 amino acids from the C-terminal region of human IMP3.

Molecular Weight: 21850 DA

Comments:

Background: This gene encodes the human homolog of the yeast Imp3 protein. The protein localizes to the nucleoli and interacts with the U3 snoRNP complex. The protein contains an S4 domain.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Transcription Factors, Chromatin, Signaling, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of IMP3 Antibody (C-term) (ABIN653856) in Hela cell line lysates (35ug/lane). IMP3 (arrow) was detected using the purified Pab.

IMP3 Antibody (C-term) (ABIN653856) IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IMP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

IMP3 Antibody (C-term) (ABIN653856) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Riener, Fritzsche, Clavien et al.: "IMP3 expression in lesions of the biliary tract: a marker for high-grade dysplasia and an independent prognostic factor in bile duct carcinomas." in: Human pathology, Vol. 40, Issue 10, pp. 1377-83, 2009 (PubMed).

Noske, Faggad, Wirtz et al.: "IMP3 expression in human ovarian cancer is associated with improved survival." in: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, Vol. 28, Issue 3, pp. 203-10, 2009 (PubMed).

Li, Yan, Tang et al.: "IMP3 is a novel prognostic marker that correlates with colon cancer progression and pathogenesis." in: Annals of surgical oncology, Vol. 16, Issue 12, pp. 3499-506, 2010 (PubMed).

Walter, Prasad, Lu et al.: "IMP3 is a novel biomarker for triple negative invasive mammary carcinoma associated with a more aggressive phenotype." in: Human pathology, Vol. 40, Issue 11, pp. 1528-33, 2009 (PubMed).