DEN1A (C-Term) antibody

Antigen: DEN1A

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653873

Additional Information

Gene ID: 57706

UniProt: Q8TEH3

Immunogen: This DEN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 583~613 amino acids from the C-terminal region of human DEN1A.

Isotype: Ig

Description: Other names: Protein FAM31A, Connecdenn, DENN domain-containing protein 1A,KIAA1608, FAM31A, DENND1A

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DEN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 583~613 amino acids from the C-terminal region of human DEN1A.

Molecular Weight: 110577 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Structure, Protein Modifications, Neurology

Restrictions: For Research Use only

Images

Western blot analysis of DEN1A Antibody (C-term) (ABIN653873) in K562 cell line lysates (35ug/lane). DEN1A (arrow) was detected using the purified Pab.

DEN1A Antibody (C-term) (ABIN653873) IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEN1A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

DEN1A Antibody (C-term) (ABIN653873) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Allaire, Ritter, Thomas et al.: "Connecdenn, a novel DENN domain-containing protein of neuronal clathrin-coated vesicles functioning in synaptic vesicle endocytosis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 51, pp. 13202-12, 2006 (PubMed).