Autophagy Related 13 (ATG13) (N-Term) antibody

Antigen: Autophagy Related 13 (ATG13)

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653882

Additional Information

Alternative name: KIAA0652

Gene ID: 9776

UniProt: O75143

Immunogen: This KIAA0652 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human KIAA0652.

Isotype: Ig

Description: Other names: Autophagy-related protein 13,ATG13, KIAA0652

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This ATG13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human ATG13.

Molecular Weight: 56572 DA

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Autophagy, Cell Structure

Restrictions: For Research Use only

Images

ATG13 Antibody (N-term) (ABIN653882) western blot analysis in MDA-MB435,CEM,T47D cell line and mouse cerebellum tissue lysates (35ug/lane).This demonstrates the ATG13 antibody detected the ATG13 protein (arrow).

ATG13 Antibody (N-term) (ABIN653882) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATG13 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Hosokawa, Hara, Kaizuka et al.: "Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy." in: Molecular biology of the cell, Vol. 20, Issue 7, pp. 1981-91, 2009 (PubMed).