DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 49 (DDX49) (C-Term) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 49 (DDX49)

Synonyms: FLJ10432, R27090_2, wu:fb82g04, wu:fd12e05, ddx49-a, MGC76291, r27090_2, DDX49

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN653921

Additional Information

Alternative name: DDX49

Gene ID: 54555

UniProt: Q9Y6V7

Immunogen: This DDX49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 392~421 amino acids from the C-terminal region of human DDX49.

Isotype: Ig

Description: Other names: DEAD box protein 49, Probable ATP-dependent RNA helicase DDX49,DDX49

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DDX49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 392~421 amino acids from the C-terminal region of human DDX49.

Molecular Weight: 54226 DA

Comments:

Background: The function of DDX49 has not yet been determined.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100 Flow cytometric~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Chromatin, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of DDX49 Antibody (C-term) (ABIN653921) in Hela cell line lysates (35ug/lane). DDX49 (arrow) was detected using the purified Pab.

DDX49 Antibody (C-term) (ABIN653921) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DDX49 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

DDX49 Antibody (C-term) (ABIN653921) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Grimwood, Gordon, Olsen et al.: "The DNA sequence and biology of human chromosome 19." in: Nature, Vol. 428, Issue 6982, pp. 529-35, 2004 (PubMed).