DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11) (Center) antibody

Antigen: DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11)

Synonyms: CHL1, KRG2, CHLR1, MGC9335, MGC133249

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654411

Additional Information

Alternative name: DDX11

Gene ID: 1663

UniProt: NP_689651.1, NP_004390.3, Q96FC9

Immunogen: This DDX11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 663-691 amino acids from the Central region of human DDX11.

Isotype: Ig

Description: Other names: DDX11,Probable ATP-dependent RNA helicase DDX11,CHL1, CHLR1, KRG2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DDX11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 663-691 amino acids from the Central region of human DDX11.

Molecular Weight: 108313 DA

Comments:

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX11 encodes a DEAD box protein, which is an enzyme that possesses both ATPase and DNA helicase activities. DDX11 is a homolog of the yeast CHL1 gene, and may function to maintain chromosome transmission fidelity and genome stability.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Transcription Factors, Signaling, Chromatin, Metabolism, Cell Structure

Restrictions: For Research Use only

Images

DDX11 Antibody (Center) (ABIN654411) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane). This demonstrates the DDX11 antibody detected the DDX11 protein (arrow).

Publications

Product: Parish, Rosa, Wang et al.: "The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells." in: Journal of cell science, Vol. 119, Issue Pt 23, pp. 4857-65, 2006 (PubMed).

Parish, Bean, Park et al.: "ChlR1 is required for loading papillomavirus E2 onto mitotic chromosomes and viral genome maintenance." in: Molecular cell, Vol. 24, Issue 6, pp. 867-76, 2006 (PubMed).

Farina, Shin, Kim et al.: "Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1." in: The Journal of biological chemistry, Vol. 283, Issue 30, pp. 20925-36, 2008 (PubMed).

Leman, Noguchi, Lee et al.: "Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion." in: Journal of cell science, Vol. 123, Issue Pt 5, pp. 660-70, 2010 (PubMed).