Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B) (C-Term) antibody

Antigen: Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B)

Synonyms: 5PTase, MGC65156, MGC71303

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654447

Additional Information

Alternative name: INPP5B

Gene ID: 3633

UniProt: P32019, NP_005531.2

Immunogen: This INPP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 957-987 amino acids from the C-terminal region of human INPP5B.

Isotype: Ig

Description: Other names: INPP5B,Type II inositol-1,4,5-trisphosphate 5-phosphatase,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This INPP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 957-987 amino acids from the C-terminal region of human INPP5B.

Molecular Weight: 112852 DA

Comments:

Background: Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in response to extracellular signals. The IP signaling molecules are inactivated by a family of inositol polyphosphate-5-phosphatases (5-phosphatases). INPP5B encodes the type II 5-phosphatase. The protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Jänne, Rochelle, Martin-DeLeon et al.: "Mapping of the 75-kDa inositol polyphosphate-5-phosphatase (Inpp5b) to distal mouse chromosome 4 and its exclusion as a candidate gene for dysgenetic lens." in: Genomics, Vol. 28, Issue 2, pp. 280-5, 1996 (PubMed).

Williams, Choudhury, McKenzie et al.: "Targeting of the type II inositol polyphosphate 5-phosphatase INPP5B to the early secretory pathway." in: Journal of cell science, Vol. 120, Issue Pt 22, pp. 3941-51, 2007 (PubMed).

Mao, Balkin, Zoncu et al.: "A PH domain within OCRL bridges clathrin-mediated membrane trafficking to phosphoinositide metabolism." in: The EMBO journal, Vol. 28, Issue 13, pp. 1831-42, 2009 (PubMed).

Coon, Mukherjee, Hanna et al.: "Lowe syndrome patient fibroblasts display Ocrl1-specific cell migration defects that cannot be rescued by the homologous Inpp5b phosphatase." in: Human molecular genetics, Vol. 18, Issue 23, pp. 4478-91, 2009 (PubMed).