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Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2) (Center) antibody
|Synonyms||SREBP2, bHLHd2, SREBP-2, AI608257, SREBP2gc, MGC124823, Srebf2_retired, SREBF2, srebp2, zgc:158371|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|3 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402-431 amino acids from the Central region of human SREBF2.|
|Description||Other names: Sterol regulatory element-binding protein 2,SREBF2,BHLHD2, SREBP2|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402-431 amino acids from the Central region of human SREBF2.|
|Molecular Weight||123688 DA|
Background: SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol. LS-B1609 polyclonal antibody detects both precursor and active forms of the protein in tissues and cells such as liver, brown fat, testis, Hep-G2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500 Immunohistochemistry ~~ 1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Translation Factors, Phospho-specific antibodies, Cell Structure, Protein Modifications, Transcription Factors, Signaling, Metabolism|
|Restrictions||For Research Use only|
|Confocal immunofluorescent analysis of SREBF2 Antibody (Center) (ABIN654630) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).|
Najafi-Shoushtari, Kristo, Li et al.: "MicroRNA-33 and the SREBP host genes cooperate to control cholesterol homeostasis." in: Science (New York, N.Y.), Vol. 328, Issue 5985, pp. 1566-9, 2010 (PubMed).
Marquart, Allen, Ory et al.: "miR-33 links SREBP-2 induction to repression of sterol transporters." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 27, pp. 12228-32, 2010 (PubMed).
Gerin, Clerbaux, Haumont et al.: "Expression of miR-33 from an SREBP2 intron inhibits cholesterol export and fatty acid oxidation." in: The Journal of biological chemistry, Vol. 285, Issue 44, pp. 33652-61, 2010 (PubMed).
|Reactivities||Human (31), Mouse (Murine) (23), Rat (Rattus) (23), Pig (Porcine) (20), Horse (Equine) (18), Chicken (2), Dog (Canine) (2), Rabbit (1), Xenopus laevis (1)|
|Applications||Immunofluorescence (IF) (16), Western Blotting (WB) (16), ELISA (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||Center (2), Internal Region (2), AA 455-469 (1), AA 580-640 (1)|