H2A Histone Family, Member Y2 (H2AFY2) (N-Term) antibody

Antigen: H2A Histone Family, Member Y2 (H2AFY2)

Synonyms: macroH2A2, RGD1561371, MGC69037, MGC130768, macroh2a2, MGC130227, MGC130228, MGC139428, MGC114051, im:6898017, wu:fa14g05, zgc:114051

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654683

Additional Information

Alternative name: H2AFY2

Gene ID: 55506

UniProt: Q9P0M6, NP_061119.1

Immunogen: This H2AFY2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human H2AFY2.

Isotype: Ig

Description: Other names: Core histone macro-H2A.2,H2AFY2,MACROH2A2

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This H2AFY2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human H2AFY2.

Molecular Weight: 40058 DA

Comments:

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

H2AFY2 Antibody (N-term) (ABIN654683) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Publications

Product: Deloukas, Earthrowl, Grafham et al.: "The DNA sequence and comparative analysis of human chromosome 10." in: Nature, Vol. 429, Issue 6990, pp. 375-81, 2004 (PubMed).

Sporn, Kustatscher, Hothorn et al.: "Histone macroH2A isoforms predict the risk of lung cancer recurrence." in: Oncogene, Vol. 28, Issue 38, pp. 3423-8, 2009 (PubMed).

Xu, Zheng, Isaacs et al.: "Inherited genetic variant predisposes to aggressive but not indolent prostate cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 5, pp. 2136-40, 2010 (PubMed).