Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1) (C-Term) antibody

Antigen: Nuclear Factor (erythroid-Derived 2)-Like 1 (NFE2L1)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN654881

Additional Information

Alternative name: NFE2L1

Gene ID: 4779

UniProt: NP_003195.1, Q14494

Immunogen: This NFE2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 712-742 amino acids from the C-terminal region of human NFE2L1.

Isotype: Ig

Description: Other names: Nuclear factor erythroid 2-related factor 1,NFE2L1,HBZ17, NRF1, TCF11

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NFE2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 712-742 amino acids from the C-terminal region of human NFE2L1.

Molecular Weight: 84704 DA

Comments:

Background: This gene encodes a protein that is involved in globin gene expression in erythrocytes. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene, NFE2L1, and for 'nuclear respiratory factor 1' which has an official symbol of NRF1.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

NFE2L1 Antibody (C-term) (ABIN654881) western blot analysis in mouse lung tissue lysates (35ug/lane). This demonstrates the NFE2L1 antibody detected the NFE2L1 protein (arrow).

Publications

Product: Newman, Keating: "Comprehensive identification of human bZIP interactions with coiled-coil arrays." in: Science (New York, N.Y.), Vol. 300, Issue 5628, pp. 2097-101, 2003 (PubMed).

Wang, Kwok, Chan: "The p65 isoform of Nrf1 is a dominant negative inhibitor of ARE-mediated transcription." in: The Journal of biological chemistry, Vol. 282, Issue 34, pp. 24670-8, 2007 (PubMed).

Radhakrishnan, Lee, Young et al.: "Transcription factor Nrf1 mediates the proteasome recovery pathway after proteasome inhibition in mammalian cells." in: Molecular cell, Vol. 38, Issue 1, pp. 17-28, 2010 (PubMed).

Steffen, Seeger, Koch et al.: "Proteasomal degradation is transcriptionally controlled by TCF11 via an ERAD-dependent feedback loop." in: Molecular cell, Vol. 40, Issue 1, pp. 147-58, 2010 (PubMed).