Lysyl-tRNA Synthetase (KARS) (N-Term) antibody

Antigen: Lysyl-tRNA Synthetase (KARS)

Synonyms: KRS, KARS2, KIAA0070, CG12141, DmelCG12141, LysRS, AA589550, AL024334, AL033315, AL033367, D8Wsu108e, mKIAA0070, D8Ertd698e, cb530, zgc:92483, kars, MGC53345, krs, kars2, krs-1, MGC52992, KARS, MGC127504, DKFZp468A0216, MGC94484, MGC69375, Tb06.28P18.190

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655049

Additional Information

Alternative name: KARS

Gene ID: 3735

UniProt: Q15046, NP_005539.1, NP_001123561.1

Immunogen: This KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human KARS.

Isotype: Ig

Description: Other names: Lysyl-tRNA synthetase,KARS,KIAA0070

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the N-terminal region of human KARS.

Molecular Weight: 68048 DA

Comments:

Background: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms: KRS, KARS2, KIAA0070, CG12141, DmelCG12141, LysRS, AA589550, AL024334, AL033315, AL033367, D8Wsu108e, mKIAA0070, D8Ertd698e, cb530, zgc:92483, kars, MGC53345, krs, kars2, krs-1, MGC52992, KARS, MGC127504, DKFZp468A0216, MGC94484, MGC69375, Tb06.28P18.190

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Chromatin

Restrictions: For Research Use only

Publications

Product: Kawamata, Magrané, Kunst et al.: "Lysyl-tRNA synthetase is a target for mutant SOD1 toxicity in mitochondria." in: The Journal of biological chemistry, Vol. 283, Issue 42, pp. 28321-8, 2008 (PubMed).

McLaughlin, Sakaguchi, Liu et al.: "Compound heterozygosity for loss-of-function lysyl-tRNA synthetase mutations in a patient with peripheral neuropathy." in: American journal of human genetics, Vol. 87, Issue 4, pp. 560-6, 2010 (PubMed).