Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (C-Term) antibody

Antigen: Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1)

Synonyms: GAD, SCP, FLJ45882, EP10, GAD25, GAD44, GAD67, Gad-1, Gad67, gad1, gad1-A, GAD1, GAD-67, MGC142602, GLUTAMATE DECARBOXYLASE, GLUTAMATE DECARBOXYLASE 1, MKP11.30, MKP11_30, DKFZp459M0324

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655175

Additional Information

Alternative name: GAD1

Gene ID: 2571

UniProt: NP_000808, Q99259

Immunogen: This GAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-551 amino acids from the C-terminal region of human GAD1.

Isotype: Ig

Description: Other names: GAD1,Glutamate decarboxylase 1,GAD, GAD67

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-551 amino acids from the C-terminal region of human GAD1.

Molecular Weight: 66897 DA

Comments:

Background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Metabolism

Restrictions: For Research Use only

Images

GAD1 Antibody (C-term) (ABIN655175) western blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the GAD1 antibody detected the GAD1 protein (arrow).

Publications

Product: Ruaño, Thompson, Kane et al.: "Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?" in: Pharmacogenomics, Vol. 11, Issue 7, pp. 959-71, 2010 (PubMed).