HAUS Augmin-Like Complex, Subunit 3 (HAUS3) (Center) antibody

Antigen: HAUS Augmin-Like Complex, Subunit 3 (HAUS3)

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655250

Additional Information

Alternative name: HAUS3

Gene ID: 79441

UniProt: Q68CZ6, NP_078787.2

Immunogen: This HAUS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-408 amino acids from the Central region of human HAUS3.

Isotype: Ig

Description: Other names: HAUS3,HAUS augmin-like complex subunit 3,C4orf15

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HAUS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-408 amino acids from the Central region of human HAUS3.

Molecular Weight: 69650 DA

Comments:

Background: HAUS3 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220], Uehara et al., 2009 [PubMed 19369198]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Cell Cycle

Restrictions: For Research Use only

Images

HAUS3 Antibody (Center) (ABIN655250) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HAUS3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Goshima, Mayer, Zhang et al.: "Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle." in: The Journal of cell biology, Vol. 181, Issue 3, pp. 421-9, 2008 (PubMed).

Shah, Morin, Khattra et al.: "Mutational evolution in a lobular breast tumour profiled at single nucleotide resolution." in: Nature, Vol. 461, Issue 7265, pp. 809-13, 2009 (PubMed).