POU Domain, Class 5, Transcription Factor 1 (POU5F1) (Cys279) antibody

Antigen: POU Domain, Class 5, Transcription Factor 1 (POU5F1)

Synonyms: gp9, gp-9, oct4, pou2, POU-2, cb197, spg/pou2, chunp6868, wu:fd18d06, POU5F1, OCT3, OTF3, OTF4, Oct4

Binding Site: Cys279

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655493

Additional Information

Alternative name: OCT3/4

Gene ID: 5460

UniProt: NP_002692.2, Q01860

Immunogen: This POU5F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-305 amino acids from the human POU5F1.

Isotype: Ig

Description: Other names: POU domain, class 5, transcription factor 1,POU5F1,OCT3, OCT4, OTF3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This POU5F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-305 amino acids from the human POU5F1.

Molecular Weight: 38571 DA

Comments:

Background: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Signaling, Protein Modifications, Transcription Factors, Ubiquitin-related antibodies

Restrictions: For Research Use only

Images

OCT3/4 Antibody (C279) (ABIN655493) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OCT3/4 Antibody (C279) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Wang, Zhao, Xiao et al.: "Alternative translation of OCT4 by an internal ribosome entry site and its novel function in stress response." in: Stem cells (Dayton, Ohio), Vol. 27, Issue 6, pp. 1265-75, 2009 (PubMed).

Kim, Lim, Kim: "EWS-Oct-4B, an alternative EWS-Oct-4 fusion gene, is a potent oncogene linked to human epithelial tumours." in: British journal of cancer, Vol. 102, Issue 2, pp. 436-46, 2010 (PubMed).

Ucisik-Akkaya, Davis, Do et al.: "Examination of genetic polymorphisms in newborns for signatures of sex-specific prenatal selection." in: Molecular human reproduction, Vol. 16, Issue 10, pp. 770-7, 2010 (PubMed).

Schultz, Desbordes, Du et al.: "Single-molecule analysis reveals changes in the DNA replication program for the POU5F1 locus upon human embryonic stem cell differentiation." in: Molecular and cellular biology, Vol. 30, Issue 18, pp. 4521-34, 2010 (PubMed).

Abu-Remaileh, Gerson, Farago et al.: "Oct-3/4 regulates stem cell identity and cell fate decisions by modulating Wnt/β-catenin signalling." in: The EMBO journal, Vol. 29, Issue 19, pp. 3236-48, 2010 (PubMed).