|+1 404 474 4654|
|+1 888 205 9894 (TF)|
POU Domain, Class 5, Transcription Factor 1 (POU5F1) (Cys279) antibody
|Synonyms||gp9, gp-9, oct4, pou2, POU-2, cb197, spg/pou2, chunp6868, wu:fd18d06, POU5F1, OCT3, OTF3, OTF4, Oct4|
Alternatives Western Blotting (WB), ELISA
|5 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This POU5F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-305 amino acids from the human POU5F1.|
|Description||Other names: POU domain, class 5, transcription factor 1,POU5F1,OCT3, OCT4, OTF3|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This POU5F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-305 amino acids from the human POU5F1.|
|Molecular Weight||38571 DA|
Background: This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Cell Structure, Signaling, Protein Modifications, Transcription Factors, Ubiquitin-related antibodies|
|Restrictions||For Research Use only|
Wang, Zhao, Xiao et al.: "Alternative translation of OCT4 by an internal ribosome entry site and its novel function in stress response." in: Stem cells (Dayton, Ohio), Vol. 27, Issue 6, pp. 1265-75, 2009 (PubMed).
Kim, Lim, Kim: "EWS-Oct-4B, an alternative EWS-Oct-4 fusion gene, is a potent oncogene linked to human epithelial tumours." in: British journal of cancer, Vol. 102, Issue 2, pp. 436-46, 2010 (PubMed).
Ucisik-Akkaya, Davis, Do et al.: "Examination of genetic polymorphisms in newborns for signatures of sex-specific prenatal selection." in: Molecular human reproduction, Vol. 16, Issue 10, pp. 770-7, 2010 (PubMed).
Schultz, Desbordes, Du et al.: "Single-molecule analysis reveals changes in the DNA replication program for the POU5F1 locus upon human embryonic stem cell differentiation." in: Molecular and cellular biology, Vol. 30, Issue 18, pp. 4521-34, 2010 (PubMed).
Abu-Remaileh, Gerson, Farago et al.: "Oct-3/4 regulates stem cell identity and cell fate decisions by modulating Wnt/β-catenin signalling." in: The EMBO journal, Vol. 29, Issue 19, pp. 3236-48, 2010 (PubMed).
|Hosts||Rabbit (26), Mouse (8), Goat (3)|
|Reactivities||Human (30), Cow (Bovine) (4), Dog (Canine) (3), Mouse (Murine) (3), Rabbit (3), Rat (Rattus) (3), Chimpanzee (2), Pig (Porcine) (2)|
|Applications||Western Blotting (WB) (26), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), ELISA (11), Immunofluorescence (IF) (4), Immunohistochemistry (IHC) (3), Flow Cytometry (FACS) (1), Immunoprecipitation (IP) (1)|
|Epitopes||AA 100-200 (1), AA 230-244 (1), AA 232-236 (1), N-Term (1), pSer236 (1)|