Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1) (Center) antibody

Antigen: Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1)

Synonyms: hexim1, hexim, MGC115232, Clp1, HIS1, CLP-1, 7330426E13Rik, HEXIM1, Clp-1, MGC143004, fd44h02, MGC162976, wu:fd44h02, zgc:162976

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655589

Additional Information

Alternative name: HEXIM1

Gene ID: 10614

UniProt: O94992, NP_006451.1

Immunogen: This HEXIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from the Central region of human HEXIM1.

Isotype: Ig

Description: Other names: Protein HEXIM1,HEXIM1,CLP1, EDG1, HIS1, MAQ1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HEXIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from the Central region of human HEXIM1.

Molecular Weight: 40623 DA

Comments:

Background: Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Structure, Signaling, Protein Modifications, Phospho-specific antibodies, Transcription Factors, Translation Factors

Restrictions: For Research Use only

Images

HEXIM1 antibody (Center) (ABIN655589) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HEXIM1 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Publications

Product: Dow, Liu, Rice: "T-loop phosphorylated Cdk9 localizes to nuclear speckle domains which may serve as sites of active P-TEFb function and exchange between the Brd4 and 7SK/HEXIM1 regulatory complexes." in: Journal of cellular physiology, Vol. 224, Issue 1, pp. 84-93, 2010 (PubMed).

Schönichen, Bigalke, Urbanke et al.: "A flexible bipartite coiled coil structure is required for the interaction of Hexim1 with the P-TEFB subunit cyclin T1." in: Biochemistry, Vol. 49, Issue 14, pp. 3083-91, 2010 (PubMed).

Ogba, Doughman, Chaplin et al.: "HEXIM1 modulates vascular endothelial growth factor expression and function in breast epithelial cells and mammary gland." in: Oncogene, Vol. 29, Issue 25, pp. 3639-49, 2010 (PubMed).