Interferon Regulatory Factor 9 (IRF9) (N-Term) antibody

Antigen: Interferon Regulatory Factor 9 (IRF9)

Synonyms: p48, IRF-9, ISGF3, ISGF3G

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655818

Additional Information

Alternative name: IRF9

Gene ID: 10379

UniProt: Q00978, NP_006075

Immunogen: This IRF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-112 amino acids from the N-terminal region of human IRF9.

Isotype: Ig

Description: Other names: Interferon regulatory factor 9,IRF9,ISGF3G

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This IRF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-112 amino acids from the N-terminal region of human IRF9.

Molecular Weight: 43696 DA

Comments:

Background: IRF9 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Mosbruger, Duggal, Goedert et al.: "Large-scale candidate gene analysis of spontaneous clearance of hepatitis C virus." in: The Journal of infectious diseases, Vol. 201, Issue 9, pp. 1371-80, 2010 (PubMed).

Jugessur, Shi, Gjessing et al.: "Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia." in: PLoS ONE, Vol. 5, Issue 7, pp. e11493, 2010 (PubMed).