Kruppel-Like Factor 4 (Gut) (KLF4) (C-Term) antibody

Antigen: Kruppel-Like Factor 4 (Gut) (KLF4)

Synonyms: EZF, GKLF, Zie, Gklf, MGC93286, KLF4, MGC139189

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN655898

Additional Information

Alternative name: KLF4

Gene ID: 9314

UniProt: O43474, NP_004226

Immunogen: This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 357-386 amino acids from the C-terminal region of human KLF4.

Isotype: Ig

Description: Other names: KLF4,Krueppel-like factor 4,EZF, GKLF

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This KLF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 357-386 amino acids from the C-terminal region of human KLF4.

Molecular Weight: 54671 DA

Comments:

Background: KLF4 is a transcription factor which acts as both an activator and repressor. Binds the CACCC core sequence. Binds to multiple sites in the 5'-flanking region of its own gene and can activate its own transcription. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

KLF4 Antibody (C-term) (ABIN655898) western blot analysis in K562 cell line lysates (35ug/lane). This demonstrates the KLF4 antibody detected the KLF4 protein (arrow).

Publications

Product: Guan, Xie, Leithäuser et al.: "KLF4 is a tumor suppressor in B-cell non-Hodgkin lymphoma and in classic Hodgkin lymphoma." in: Blood, Vol. 116, Issue 9, pp. 1469-78, 2010 (PubMed).

Ohnesorge, Viemann, Schmidt et al.: "Erk5 activation elicits a vasoprotective endothelial phenotype via induction of Kruppel-like factor 4 (KLF4)." in: The Journal of biological chemistry, Vol. 285, Issue 34, pp. 26199-210, 2010 (PubMed).

Wong, Hou, Tseng et al.: "Krüppel-like transcription factor 4 contributes to maintenance of telomerase activity in stem cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 9, pp. 1510-7, 2010 (PubMed).