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Mediator Complex Subunit 6 (MED6) (C-Term) antibody
Alternatives Western Blotting (WB), ELISA
|5 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This MED6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-246 amino acids from the C-terminal region of human MED6.|
|Description||Other names: MED6,Mediator of RNA polymerase II transcription subunit 6,ARC33|
|Characteristics||Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This MED6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-246 amino acids from the C-terminal region of human MED6.|
|Molecular Weight||28425 DA|
Background: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
|Application Notes||The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Translation Factors, Signaling, Protein Modifications, Transcription Factors, Cell Structure|
|Restrictions||For Research Use only|
|MED6 Antibody (C-term) (ABIN656028) western blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the MED6 antibody detected the MED6 protein (arrow).|
Sato, Tomomori-Sato, Banks et al.: "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex." in: The Journal of biological chemistry, Vol. 278, Issue 50, pp. 49671-4, 2003 (PubMed).
Tomomori-Sato, Sato, Parmely et al.: "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5846-51, 2004 (PubMed).
Yang, DeBeaumont, Zhou et al.: "The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 8, pp. 2339-44, 2004 (PubMed).
Sato, Tomomori-Sato, Parmely et al.: "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." in: Molecular cell, Vol. 14, Issue 5, pp. 685-91, 2004 (PubMed).
Pavri, Lewis, Kim et al.: "PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator." in: Molecular cell, Vol. 18, Issue 1, pp. 83-96, 2005 (PubMed).
|Hosts||Rabbit (13), Mouse (3)|
|Reactivities||Human (16), Mouse (Murine) (6), Rat (Rattus) (6), Chicken (3), Cow (Bovine) (3), Dog (Canine) (3), Cat (Feline) (1)|
|Applications||Western Blotting (WB) (16), ELISA (13), Immunohistochemistry (IHC) (2), Immunofluorescence (IF) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1), Immunoprecipitation (IP) (1)|
|Epitopes||Center (2), Internal Region (2), C-Term (1), C-Term,AA 214-246 (1), Internal Region,AA 134-164 (1)|