Mediator Complex Subunit 6 (MED6) (C-Term) antibody

Antigen: Mediator Complex Subunit 6 (MED6)

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656028

Additional Information

Alternative name: MED6

Gene ID: 10001

UniProt: NP_005457.2, O75586

Immunogen: This MED6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-246 amino acids from the C-terminal region of human MED6.

Isotype: Ig

Description: Other names: MED6,Mediator of RNA polymerase II transcription subunit 6,ARC33

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MED6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-246 amino acids from the C-terminal region of human MED6.

Molecular Weight: 28425 DA

Comments:

Background: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

MED6 Antibody (C-term) (ABIN656028) western blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the MED6 antibody detected the MED6 protein (arrow).

Publications

Product: Sato, Tomomori-Sato, Banks et al.: "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex." in: The Journal of biological chemistry, Vol. 278, Issue 50, pp. 49671-4, 2003 (PubMed).

Tomomori-Sato, Sato, Parmely et al.: "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5846-51, 2004 (PubMed).

Yang, DeBeaumont, Zhou et al.: "The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 8, pp. 2339-44, 2004 (PubMed).

Sato, Tomomori-Sato, Parmely et al.: "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology." in: Molecular cell, Vol. 14, Issue 5, pp. 685-91, 2004 (PubMed).

Pavri, Lewis, Kim et al.: "PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator." in: Molecular cell, Vol. 18, Issue 1, pp. 83-96, 2005 (PubMed).