Mediator Complex Subunit 6 (MED6) (Middle Region) antibody

Details for Product No. ABIN2778336, Supplier: Log in to see
Antigen
  • NY-REN-28
  • wu:fa14c11
  • zgc:86933
  • 1500012F11Rik
  • AU019660
  • AV213303
  • RNA polymerase transcriptional regulation mediator-like protein
  • mediator complex subunit 6
  • hypothetical protein
  • MED6
  • Med6
  • med6
  • CAALFM_C306170CA
Alternatives
anti-Human Mediator Complex Subunit 6 antibody for ELISA
Epitope
Middle Region
15
10
10
10
8
8
8
7
6
2
2
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
80
18
16
6
5
5
4
4
3
2
1
1
1
1
1
Host
Rabbit
67
14
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
4
4
4
Application
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
80
43
5
3
2
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human MED6
Blocking Peptide Blocking peptide for this product available: ABIN979955
Sequence LLLDLRQKFP PKFVQLKPGE KPVPVDQTKK EAEPIPETVK PEEKETTKNV
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 93%, Rat: 93%
Characteristics This is a rabbit polyclonal antibody against MED6. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name MED6 (MED6 Antibody Abstract)
Background MED6 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. And mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Alias Symbols: NY-REN-28
Protein Interaction Partner: TARDBP, UBC, CDK19, CDK8, MED10, MED23, CCNC, MED19, MED26, FBXW7, MED11, MED28, MED29, MED4, MED12, MED7, MED21, MED1, SMARCA4, BCL6, SUZ12, CUL1, SKP1, CTDP1, MED25, SREBF1, MED15, VDR, OBFC1, MED18, ZC3H13, TADA2A, RARA, PARP1, SMAD3, SMAD2, SMAD1, MED
Protein Size: 246
Molecular Weight 28 kDa
Gene ID 10001
NCBI Accession NM_005466, NP_005457
UniProt O75586
Pathways Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 246 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Mediator Complex Subunit 6 (MED6) (Middle Region) antibody (ABIN2778336) WB Suggested Anti-MED6 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Posi...
Western Blotting (WB) image for anti-Mediator Complex Subunit 6 (MED6) (Middle Region) antibody (ABIN2778336) Host: Rabbit Target Name: MED6 Sample Type: Human Fetal Muscle Antibody Dilutio...
Western Blotting (WB) image for anti-Mediator Complex Subunit 6 (MED6) (Middle Region) antibody (ABIN2778336) Host: Rabbit Target Name: MED6 Sample Type: Human Fetal Brain Antibody Dilution...
Chromatin Immunoprecipitation (ChIP) image for anti-Mediator Complex Subunit 6 (MED6) (Middle Region) antibody (ABIN2778336) Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three t...
Background publications Pavri, Lewis, Kim, Dilworth, Erdjument-Bromage, Tempst, de Murcia, Evans, Chambon, Reinberg: "PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator." in: Molecular cell, Vol. 18, Issue 1, pp. 83-96, 2005 (PubMed).

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