Naked Cuticle Homolog 2 (Drosophila) (NKD2) (C-Term) antibody

Antigen: Naked Cuticle Homolog 2 (Drosophila) (NKD2)

Synonyms: AW212591, 2210403L10Rik, Naked2, nkd2b

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656092

Additional Information

Alternative name: NKD2

Gene ID: 85409

UniProt: NP_149111.1, Q969F2

Immunogen: This NKD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-401 amino acids from the C-terminal region of human NKD2.

Isotype: Ig

Description: Other names: NKD2,Protein naked cuticle homolog 2,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NKD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-401 amino acids from the C-terminal region of human NKD2.

Molecular Weight: 50055 DA

Comments:

Background: In the mouse, Nkd is a Dishevelled (see DVL1, MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1, MIM 164820)-beta-catenin (see MIM 116806)-Tcf (see MIM 602272) signaling pathway.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Ubiquitin-related antibodies, Cell Signaling, Cell Structure, Protein Modifications

Restrictions: For Research Use only

Publications

Product: Li, Franklin, Graves-Deal et al.: "Myristoylated Naked2 escorts transforming growth factor alpha to the basolateral plasma membrane of polarized epithelial cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 15, pp. 5571-6, 2004 (PubMed).

Adamowicz, Radlwimmer, Rieker et al.: "Frequent amplifications and abundant expression of TRIO, NKD2, and IRX2 in soft tissue sarcomas." in: Genes, chromosomes & cancer, Vol. 45, Issue 9, pp. 829-38, 2006 (PubMed).

Li, Hao, Cao et al.: "Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha containing exocytic vesicles at the lower lateral membrane of polarized MDCK cells." in: Molecular biology of the cell, Vol. 18, Issue 8, pp. 3081-93, 2007 (PubMed).

Ding, Li, Hu et al.: "EGF receptor-independent action of TGF-alpha protects Naked2 from AO7-mediated ubiquitylation and proteasomal degradation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 36, pp. 13433-8, 2008 (PubMed).

Hu, Li, Cao et al.: "Myristoylated Naked2 antagonizes Wnt-beta-catenin activity by degrading Dishevelled-1 at the plasma membrane." in: The Journal of biological chemistry, Vol. 285, Issue 18, pp. 13561-8, 2010 (PubMed).