Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2) (Center) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2)

Synonyms: GCK, BL44, RAB8IP

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656275

Additional Information

Alternative name: MAP4K2

Gene ID: 5871

UniProt: Q12851, NP_004570.2

Immunogen: This MAP4K2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-349 amino acids from the Central region of human MAP4K2.

Isotype: Ig

Description: Other names: MAP4K2,Mitogen-activated protein kinase kinase kinase kinase 2,GCK, RAB8IP

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MAP4K2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-349 amino acids from the Central region of human MAP4K2.

Molecular Weight: 91556 DA

Comments:

Background: The protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: MAP4K2 Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Chromatin, Signaling

Restrictions: For Research Use only

Images

MAP4K2 Antibody (Center) (ABIN656275) western blot analysis in Jurkat cell line lysates (35ug/lane). This demonstrates the MAP4K2 antibody detected the MAP4K2 protein (arrow).

Publications

Product: Chadee, Yuasa, Kyriakis: "Direct activation of mitogen-activated protein kinase kinase kinase MEKK1 by the Ste20p homologue GCK and the adapter protein TRAF2." in: Molecular and cellular biology, Vol. 22, Issue 3, pp. 737-49, 2002 (PubMed).

Fidalgo, Fraile, Pires et al.: "CCM3/PDCD10 stabilizes GCKIII proteins to promote Golgi assembly and cell orientation." in: Journal of cell science, Vol. 123, Issue Pt 8, pp. 1274-84, 2010 (PubMed).