Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3) (C-Term) antibody

Antigen: Ficolin (Collagen/fibrinogen Domain Containing) 3 (Hakata Antigen) (FCN3)

Synonyms: FCNH, HAKA1, MGC22543

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656492

Additional Information

Alternative name: FCN3

Gene ID: 8547

UniProt: O75636, NP_775628.1, NP_003656.2

Immunogen: This FCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-243 amino acids from the C-terminal region of human FCN3.

Isotype: Ig

Description: Other names: Ficolin-3,FCN3,FCNH, HAKA1

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This FCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-243 amino acids from the C-terminal region of human FCN3.

Molecular Weight: 32903 DA

Comments:

Background: Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum, there are two types of ficolins, both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein, which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus, has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants, each encoding a distinct isoform, have been identified.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: FCN3 Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Munthe-Fog, Hummelshøj, Ma et al.: "Characterization of a polymorphism in the coding sequence of FCN3 resulting in a Ficolin-3 (Hakata antigen) deficiency state." in: Molecular immunology, Vol. 45, Issue 9, pp. 2660-6, 2008 (PubMed).

Lacroix, Dumestre-Pérard, Schoehn et al.: "Residue Lys57 in the collagen-like region of human L-ficolin and its counterpart Lys47 in H-ficolin play a key role in the interaction with the mannan-binding lectin-associated serine proteases and the collectin receptor calreticulin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 1, pp. 456-65, 2008 (PubMed).

Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).