Regulatory Factor X 3 (RFX3) (C-Term) antibody

Antigen: Regulatory Factor X 3 (RFX3)

Synonyms: MGC87155, bA32F11.1, ba32f11.1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN656557

Additional Information

Alternative name: RFX3

Gene ID: 5991

UniProt: NP_002910.1, P48380, NP_602304.1

Immunogen: This RFX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 660-690 amino acids from the C-terminal region of human RFX3.

Isotype: Ig

Description: Other names: Transcription factor RFX3,RFX3,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RFX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 660-690 amino acids from the C-terminal region of human RFX3.

Molecular Weight: 83530 DA

Comments:

Background: This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Two transcript variants encoding different isoforms have been described for this gene, and at least one of the variants utilizes alternative polyadenylation signals.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: RFX3 Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Translation Factors, Cell Cycle, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

RFX3 Antibody (C-term) (ABIN656557) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the RFX3 antibody detected the RFX3 protein (arrow).

Publications

Product: Morotomi-Yano, Yano, Saito et al.: "Human regulatory factor X 4 (RFX4) is a testis-specific dimeric DNA-binding protein that cooperates with other human RFX members." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 836-42, 2002 (PubMed).

Humphray, Oliver, Hunt et al.: "DNA sequence and analysis of human chromosome 9." in: Nature, Vol. 429, Issue 6990, pp. 369-74, 2004 (PubMed).

El Zein, Ait-Lounis, Morlé et al.: "RFX3 governs growth and beating efficiency of motile cilia in mouse and controls the expression of genes involved in human ciliopathies." in: Journal of cell science, Vol. 122, Issue Pt 17, pp. 3180-9, 2009 (PubMed).